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Sample Discontinued
Experience: None
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Coding

Part:BBa_K908004:Design

Designed by: Wacquier Benjamin   Group: iGEM12_ULB-Brussels   (2012-09-19)


Microcin B17, Gene D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 780
    Illegal PstI site found at 813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 780
    Illegal PstI site found at 813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 906
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 780
    Illegal PstI site found at 813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 780
    Illegal PstI site found at 813
    Illegal NgoMIV site found at 1151
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design considerations


Source

Plasmid pCID909

References